Last reviewed: October 2026 · Cleared Peptides research team
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small, membrane-permeable quinolinium compound characterized as a selective inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that methylates nicotinamide using S-adenosylmethionine.
5-Amino-1MQ Structure and Identifiers
| Name | 5-Amino-1MQ |
|---|---|
| Synonyms | 5-Amino-1-methylquinolinium |
| CAS number | 42464-96-0 |
| PubChem CID | 66522933 |
| Molecular formula | C10H11N2+ (salt form per COA) |
| Molecular weight | ≈159.2 g/mol (cation) |
| Type | Small molecule (not a peptide) |
| Form | Powder |
Why NNMT Matters in Research
NNMT converts nicotinamide to 1-methylnicotinamide, diverting it from the NAD+ salvage pathway and consuming methyl groups. Inhibiting NNMT in cell models is therefore used to study NAD+ levels and methyl-donor balance — often alongside NAD+.
Published In-Vitro Research Areas
- NNMT enzyme-inhibition assays
- NAD+ and SAM metabolite measurements in adipocyte cultures
This summary describes laboratory research only and makes no claim about effects in humans or animals.
Purity Testing and Storage
Every lot of 5-Amino-1MQ supplied by Cleared Peptides is tested by an independent laboratory for identity and purity (or composition, for multi-component preparations), and the lot Certificate of Analysis is published on our COA page. Store 5-Amino-1MQ sealed at −20 °C, dry and protected from light. Handle according to your institution’s chemical-hygiene procedures.
Research materials referenced in this guide
Frequently Asked Questions
What enzyme does 5-Amino-1MQ inhibit?
Nicotinamide N-methyltransferase (NNMT).
Is 5-Amino-1MQ for human use?
No. Cleared Peptides supplies 5-Amino-1MQ for laboratory research use only; it is not for human or veterinary use.
Selected References
Peer-reviewed literature on the compounds discussed in this guide, listed as scientific background for laboratory researchers. Citation does not imply endorsement by the authors or any use other than in-vitro research.
- Ruf S, et al. Novel nicotinamide analog as inhibitor of nicotinamide N-methyltransferase. Bioorg Med Chem Lett. 2018;28(5):922-925. PubMed 29433927 · DOI
- Li P, et al. Enhancing nicotinamide N-methyltransferase bisubstrate inhibitor activity through 7-deazaadenosine and linker modifications. Bioorg Chem. 2024;143:106963. PubMed 38048700 · DOI
- Akar S, et al. Small molecule inhibitor of nicotinamide N-methyltransferase shows anti-proliferative activity in HeLa cells. J Obstet Gynaecol. 2021;41(8):1240-1245. PubMed 33645410 · DOI
Laboratory note: solubility depends on salt form and solvent. This guide does not state a single solubility figure. Handling of lyophilized material is described in the peptide storage guide.
Research Use Only Notice
This guide is provided for educational and laboratory reference purposes. It summarizes published, primarily in-vitro and structural research literature and does not describe, recommend or imply any use in humans or animals. Products sold by Cleared Peptides are for laboratory research use only; they are not drugs, foods, cosmetics or dietary supplements, are not FDA-approved, and are not intended to diagnose, treat, cure, mitigate or prevent any disease. No dosing, administration or therapeutic information is provided.
